Bioprospection of diazotrophic bacteria from lemongrass and evaluation of its potential agricultural use

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Published: Apr 30, 2026
DOI: https://doi.org/10.25061/2527-2675/ReBraM/2026.v29i1.2262
Keywords:
plantas medicinais, inoculante, óleos essenciais, fixação biológica, promotores de crescimento medicinal plants, inoclating, essencial oils, biological fixation, growth promoters plantas medicinales, inoculante, aceites esenciales, fijación biológica, promotores del crecimiento

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Patrícia Cristina de Oliveira dos Santos
Universidade Professor Edson Antônio Velano - UNIFENAS
Paulo Roberto Corrêa Landgraf
Universidade Professor Edson Antônio Velano - UNIFENAS
Ligiane Aparecida Florentino
Universidade Professor Edson Antônio Velano - UNIFENAS

Abstract

Lemon grass, a medicinal plant of economic interest, demands sustainability in the production of good quality raw materials, and resource optimization. Given this, it was aimed to evaluate the influence of diazotrophic bacteria on growth, and species development. The material collected was divided into sheet, profile, root, rhizome, and rhizospheric soil, isolated in semi -solids and semi -selective, JMV, LGI, JNFB, NFB, FAM, with three repetitions. After 15 days, the means that presented a characteristic film of the biological fixation of nitrogen were separated. The 86 isolates were grouped into a dendrogram, according to morphological characteristics, evaluating diversity, and 80% similarity, 23 strains were totaled. The experiment, conducted at a vegetation house from May 2022 to May 2023, had as treatment the 23 strains, positive control treatment, and negative control (without inoculation and without fertilization), totaling 25 treatments with 4 repetitions, in a randomized block design. The variables analyzed were green weight of the aerial part, green root weight, total weight, length length, root length, dry weight and root dry weight. All bacteria showed rapid growth and diversity among the characteristics, enabling high association capacity between the medicinal species and the various nitrogen -fixing bacterial species. The isolated BPCV, formed a microorganism collection bank, and the tested strains showed potential for use in lemon grass cultivation, replacing nitrogen fertilization, highlighting UNIFENAS 100-524, which was not higher in root growth only.

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How to Cite
Cristina de Oliveira dos Santos, P., Landgraf, P. R. C., & Florentino, L. A. (2026). Bioprospection of diazotrophic bacteria from lemongrass and evaluation of its potential agricultural use. Revista Brasileira Multidisciplinar, 29(1), 87-94. https://doi.org/10.25061/2527-2675/ReBraM/2026.v29i1.2262
Issue
Vol. 29 No. 1 (2026): Fluxo Contínuo
Section
Artigos Originais
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Author Biographies

Paulo Roberto Corrêa Landgraf, Universidade Professor Edson Antônio Velano - UNIFENAS

Forestry Engineer and Agronomist engineer from José do Rosário Vellano University, master's degree in Agronomy (Phytotechnics) from the Federal University of Lavras (1994) and PhD in Agronomy (Phytotechnics) from the Federal University of Lavras (2006). He is currently a professor at Professor Edson Antônio Velano University and coordinator of the Agronomy course. He has experience in the field of Agronomy and engineering. Forestry working mainly on the following topics: urban afforestation, floriculture, ornamental plants, seeds, recovery of degraded areas and restoration of the landscape.

Ligiane Aparecida Florentino, Universidade Professor Edson Antônio Velano - UNIFENAS

He has a degree in Agronomy, a Master's degree in Agricultural Microbiology, a PhD in Soil Science and a Post-Doctorate in Agricultural Microbiology from the Federal University of Lavras (UFLA). He has experience in the area of ​​Agronomy, with an emphasis on the use of alternatives for sustainable soil management through the use of remineralizers, green manure and inoculation with microorganisms that promote plant growth. He has also developed work in the area of ​​environmental education to raise awareness regarding the rational use of natural resources. She is currently a professor and researcher at the Professor Edson Antônio Velano University (UNIFENAS)/Fundação de Ensino e Tecnologia de Alfenas (FETA), where she participates in the Postgraduate Program in Animal Science, Professional Master's Degree in Agricultural Production Systems and Doctorate in Sustainable Agriculture .

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