Polylactic acid scaffolds obtained by 3D printing and modified by oxygen plasma

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Lorenzo Gouvêa Machado
Mayté Paredes Zaldivar
Mônica Rosas da Costa Iemma
Sandra Andrea Cruz
Elidiane Cipriano Rangel
Eduardo José Nassar
Hernane Silva Barud


The purpose of tissue engineering is to repair, replace, and regenerate tissues and organs. For this aim, materials supports, as polylactic acid (PLA) are used. PLA is a thermoplastic polymer that presents biodegradability, biocompatibility and good processability. PLA scaffolds can accurately constructed by 3D printing. Then, the objectives of this work were to modify the hydrophobic surface of PLA scaffolds using oxygen plasma and to study the cell viability and proliferation. The characterization was done by AFM, contact angle, FTIR and studies of proliferation and cell viability. Results showed that the material acquired hydrophilic properties by the presence of oxygen reactive species and by contact angle decrease. It was also observed an increase in the surface roughness. We can conclude that although the surface modifications were effective and the PLA scaffolds were not cytotoxic, there were no improvements in the proliferation process with the studied osteo-1 lineage cells. 


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Machado, L. G., Zaldivar, M. P., Iemma, M. R. da C., Cruz, S. A., Rangel, E. C., Nassar, E. J., & Barud, H. S. (2020). Polylactic acid scaffolds obtained by 3D printing and modified by oxygen plasma. Revista Brasileira Multidisciplinar, 23(1), 97-106. https://doi.org/10.25061/2527-2675/ReBraM/2020.v23i1.813
Artigos Originais
Biografia do Autor

Lorenzo Gouvêa Machado, University of Araraquara - UNIARA

Biopolymers and Biomaterials Research Group - BIOPOLMAT

Mayté Paredes Zaldivar, University of Araraquara - UNIARA

Biopolymers and Biomaterials Research Group - BIOPOLMAT

Mônica Rosas da Costa Iemma, University of Araraquara - UNIARA

Biopolymers and Biomaterials Research Group - BIOPOLMAT

Sandra Andrea Cruz, Federal University of São Carlos - UFSCAR

Department of Chemistry, Center for Exact Sciences and Technology

Elidiane Cipriano Rangel, Sorocaba Institute of Science and Technology. Paulista State University- UNESP

Plasma and Materials Group

Eduardo José Nassar, University of Franca - UNIFRAN

PhD in Chemistry from the University of São Paulo- Researcher

Hernane Silva Barud, University of Araraquara - UNIARA

Biopolymers and Biomaterials Research Group - BIOPOLMAT


BANDYOPADHYAY, A; BOSE, S. “3D printing of biomaterials”, Sumandas. MRs Bulletin, v.40, p.108-112. February, 2015.

BOSE, S; ROY,M; BANDYOPADHYAY, A. “Recent advances in bone tissue engineering scaffolds” Trends in biotechnology, v.30, n. 10, p. 546-554, 2012.

BRAGHIROLLI, D. I. – Produção de scaffolds contendo células tronco para uso na engenharia de tecidos através da associação das técnicas electrospinning e bio-eletrosparying. Julho de 2012. 94 f. Dissertação (Mestrado em Ciências dos Materiais) – Universidade Federal do Rio Grande do Sul, Porto Alegre - RS, 2014.

BRIEN, J, O; WILSON, I; ORTON, T; POGNAN, Ë. Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. European Journal of Biochemistry, v. 267, p.5421–5426, 2000.

CHEN, C. B. LIANG, D. Lu, A. OGINO, X. WANG, M. NAGATSU, “Amino group introduction onto multiwall carbon nanotubes by NH3/Ar plasma treatment”, Carbon. v. 48, [S. l.], p.939-948, 2010.

GREGOR, A; FILOVÁ, E; NOVÁK, M; KRONEK, J; CHLUP, H; BUZGO, M; BLAHNOVÁ, V; LUKÁSOVÁ, V; BARTOS, M; NECAS, A; HOSEK, J. Designing of PLA scaffolds for bone tissue replacement fabricated by ordinary commercial 3D printer. Journal of Biological Engineering, v.11, n.1, p.1–21, 2017.

INAGAKI, N.; NARUSHIMA, K.; TSUTSUI, Y.; OHYAMA, Y. Surface modification and degradation of poly(lactic acid) films by Ar-plasma. Journal of Adhesion Science and Technology, v. 16, n. 8, p. 1041–1054, 2002.

JAIDEV, L, R; CHARTTERJEE, K. Surface functionalization of 3D printed polymer scaffolds to augment stem cell response. Materials and Design, v.161, p.44–54, 2019.

JORDÁ-VILAPLANA, A; FOMBUENA, V; GARCÍA-GARCÍA, D; SAMPER, M.D; SÁNCHEZ-NÁCHER, L. Surface modification of polylactic acid (PLA) by air atmospheric plasma treatment. European Polymer Journal, v. 58, p. 23–33, 2014.

KODAMA, H. “Automatic method for fabricating a three-dimensional plastic model with photo-hardening polymer” Review of Scientific Instruments, v.52, n. 11, p. 1770–73, 1981.

LAI, Jiangnan; SUNDERLAND, Bob; XUE, Jianming; YAN, Sha; ZHAO, Weijiang; FOLKARD, Melvyn; MICHAEL, Barry D.; WANG, Yugang. Study on hydrophilicity of polymer surfaces improved by plasma treatment. Applied Surface Science, v. 252, n. 10, p. 3375–3379, 2006.

LI, J; He, L; ZHOU, C; ZHOU, Y; BAI, Y; LEE, F. Y; MAO, J. J. 3D printing for regenerative medicine: From bench to bedside. MRS Bulletin, v.40, n.2, p.145–153, 2015.

LU, L; MIKOS, A, G. The importance of new processing techniques in tissue engineering. MRS Bulletin, v. 21, n. 11, p. 28–32, Nov, 1996.

MATOS, B. D. M; ROCHA, V; DA SILVA, E. J; MORO, F. H; BOTTENE, A. C; RIBEIRO, C. A; DOS SANTOS DIAS, D; ANTONIO, S. G; DO AMARAL, A. C; CRUZ, S. A; DE OLIVEIRA BARUD, H. G; SILVA BARUD, H, da. Evaluation of commercially available polylactic acid (PLA) filaments for 3D printing applications. Journal of Thermal Analysis and Calorimetry, v.137, n.2, p.555–562, 2019.

MORENT, R; DE GEYTER, N; DESMET, T; DUBRUEL, P; LEYS, C. Plasma surface modification of biodegradable polymers: A review. Plasma Processes and Polymers, v.8, n.3, p. 171–190. 2011.

NGO, T, D; KASHANI, A; IMBALZANO, G; NGUYEN, Kate T. Q; HUI, D. Additive manufacturing (3D printing): A review of materials, methods, applications and challenges. Composites Part B: Engineering, v. 143, n.2 p.172–196, 2018.

QUEIROZ, T, S, D; PRADO, R, F; APARECIDA, I; BRITO, W, D; OLIVEIRA, L, D, D; MAROTTA, L; VASCONCELLOS, R, D; CAMARGO, E, A. Cytotoxicity and Genotoxicity of PLA and PCL Membranes on Osteoblasts. Acta Scientific Dental Sciences, v. 3, n. 4, p.55–59, 2019.

WANG, M; CHENG, X; ZHU, W; HOLMES, B; KEIDAR, M; ZHANG, L, G. Design of Biomimetic and Bioactive Cold Plasma-Modified Nanostructured Scaffolds for Enhanced Osteogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells. Tissue Engineering Part A, v. 20, n.5–6, p.1060–1071, 2013.

YEH, C, H; CHEN, Y, W; SHIE,M-Y; FANG, H-Y. “Poly (Dopamine) – Assisted Immobilization of xu Duan on 3D printed poly (lactic acid) scaffolds to up- regulate osteogenic and angiogenic markers of bone marrow stem cells. Materials, n.8, p.4299-4315, 2015.

ZEIN, I; HUTMACHER, D, W; TAN, K, C; TEOH, S, H. Fused deposition modeling of novel scaffold architectures for tissue engineering applications. Biomaterials, v.23, n.4, p.1169–1185, 2002.

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